[Loraine]

@MMMURRAY its possible that the battery is getting low, as suggest by Carol, but I’m wondering if it might be a bad tuning peg that keeps loosening causing the string to become looser and untuned. Are you saying that you use the tuner to tune the low E, but while playing it, the string becomes untuned and loosens to a D#? If that’s the case, I’m pretty sure it’s the tuning oeg. You can replace the pegs or have a luthier do it.